| | |  | | | | Record from Web of Science® | | | | | | |
| POLO ENCODES A PROTEIN-KINASE HOMOLOG REQUIRED FOR MITOSIS IN DROSOPHILA |
|
|
| Author(s): LLAMAZARES S, MOREIRA A, TAVARES A, GIRDHAM C, SPRUCE BA, GONZALEZ C, KARESS RE, GLOVER DM, SUNKEL CE |
| Source: GENES & DEVELOPMENT Volume: 5 Issue: 12A Pages: 2153-2165 Published: DEC 1991 |
| Times Cited: 208 References: 59 |
| Abstract: We show that mutation in polo leads to a variety of abnormal mitoses in Drosophila larval neuroblasts. These include otherwise normal looking mitotic spindles upon which chromosomes appear overcondensed; normal bipolar spindles with polyploid complements of chromosomes; bipolar spindles in which one pole can be unusually broad; and monopolar spindles. We have cloned the polo gene from a mutant allele carrying a P-element transposon and sequenced cDNAs corresponding to transcripts of the wild-type locus. The sequence shows that polo encodes a 577-amino-acid protein with an amino-terminal domain homologous to a serine-threonine protein kinase. polo transcripts are abundant in tissues and developmental stages in which there is extensive mitotic activity. The transcripts show no obvious spatial pattern of distribution in relation to the mitotic domains of cellularized embryos but are specifically concentrated in dividing cells in larval discs and brains. In the cell cycles of both syncytial and cellularized embryos, the polo kinase undergoes cell cycle-dependent changes in its distribution: It is predominantly cytoplasmic during interphase; it becomes associated with condensed chromosomes toward the end of prophase; and it remains associated with chromosomes until telophase, whereupon it becomes cytoplasmic. |
| Document Type: Article |
| Language: English |
| Reprint Address: LLAMAZARES, S (reprint author), UNIV DUNDEE, DEPT BIOCHEM, CELL CYCLE GENET GRP, CANC RES CAMPAIGN, DUNDEE DD1 4HN, SCOTLAND |
Addresses:
1. UNIV PORTO, INST NACL INVEST CIENTIF, CTR CITOL EXPTL, GENET MOLEC LAB, P-4100 OPORTO, PORTUGAL
2. NYU, SCH MED, DEPT BIOCHEM, NEW YORK, NY 10016 USA |
| Publisher: COLD SPRING HARBOR LAB PRESS, 1 BUNGTOWN RD, PLAINVIEW, NY 11724 |
| Subject Category: Cell Biology; Developmental Biology; Genetics & Heredity |
| IDS Number: GU846 |
| ISSN: 0890-9369 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |