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| IDENTIFICATION OF ICAM-1-POSITIVE CELLS IN THE NONGRAFTED AND TRANSPLANTED RAT-KIDNEY - AN IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL-STUDY |
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| Author(s): KANAGAWA K, ISHIKURA H, TAKAHASHI C, TAMATANI T, MIYASAKA M, TOGASHI M, KOYANAGI T, YOSHIKI T |
| Source: TRANSPLANTATION Volume: 52 Issue: 6 Pages: 1057-1062 Published: DEC 1991 |
| Times Cited: 44 References: 16 |
| Abstract: Cellular localization of intercellular adhesion molecule-1 (ICAM-1) in rat nongrafted intact kidneys and in transplanted kidneys was investigated using monoclonal anti-rat ICAM-1, 1A29. The major ICAM-1-positive cells in the nongrafted and isografted kidneys were endothelial cells in the large vessels and intertubular capillaries, as observed using light microscopy. A weak, but specific expression of ICAM-1 antigen was noted in the glomeruli, but the exact localization and cell type were not clearly discernible. In the allograft, the ICAM-1-positive cells found in the nongrafted and isografted kidneys also expressed ICAM-1 antigen. In addition, tubular epithelial cells at the luminal border and some infiltrating cells in the allograft expressed ICAM-1. In the allograft, some graft-infiltrating cells were shown to be lymphocyte function-associated antigen-1 (LFA-1)-positive. As the nature of ICAM-1-positive cells in the infiltrates was unclear, we examined ICAM-1-positive cells using immunoelectron microscopy and the direct immunoperoxidase method. Glomerular endothelial cells, podocytes, and Bowman's capsular epithelial cells expressed ICAM-1 antigen in the nongrafted and transplanted kidneys. Among the infiltrating cells in the allograft, the major ICAM-1 positive cells were macrophagelike tissues, and some blastic lymphocytes also expressed ICAM-1. Only rarely did the proximal tubular cells express ICAM-1 antigen at the luminal surfaces in the intact kidney. In the allograft, the proximal, distal, and collecting ductular epithelial cells expressed ICAM-1 at the luminal surface, and in addition, the ICAM-1 antigen was also localized at the basal surfaces of some of the renal proximal tubular epithelial cells. The upregulated ICAM-1 expression in the allograft may accelerate graft rejection by augmenting adhesiveness of LFA-1-positive graft-infiltrating cells. |
| Document Type: Article |
| Language: English |
Addresses:
1. TOKYO METROPOLITAN INST MED SCI, DEPT IMMUNOL, TOKYO 113, JAPAN
2. HOKKAIDO UNIV, SCH MED, DEPT PATHOL & UROL, SAPPORO, HOKKAIDO 060 JAPAN |
| Publisher: WILLIAMS & WILKINS, 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 |
| Subject Category: Immunology; Surgery; Transplantation |
| IDS Number: GV969 |
| ISSN: 0041-1337 |
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