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DEGREE OF POLLEN DISPERSAL BY INSECTS FROM A FIELD-TEST OF GENETICALLY ENGINEERED COTTON
Author(s): UMBECK PF, BARTON KA, NORDHEIM EV, MCCARTY JC, PARROTT WL, JENKINS JN
Source: JOURNAL OF ECONOMIC ENTOMOLOGY    Volume: 84    Issue: 6    Pages: 1943-1950    Published: DEC 1991  
Times Cited: 43     References: 13     
Abstract: This investigation was carried out to determine the movement of pollen from a field test site of genetically engineered cotton grown in 1989 at the Plant Science Research Farm of Mississippi State University. The objective was to evaluate the biosafety procedures used to reduce pollen movement. A field test of transgenic cotton measuring 136 by 30 m was bordered on all sides by 25 m of commercial cotton. All border rows were sampled from the lower, middle, and top fruiting positions of the plants. Seeds were analyzed for expression of the dominant selectable marker, neomycin phosphotransferase (npt II). Reliability of the assay was confirmed by polymerase chain reaction analysis. Results showed a consistent and significant reduction in pollen dissemination as distance from the test plot increased. Out-crossing went from 5 to < 1% by 7 m away from the test plot. A low level of pollen dispersal of < 1% continued to occur sporadically in the remaining border rows out to a distance of 25 m. No significant differences were noted for flower position on the plants, indicating there were no consistent seasonal effects on pollen dissemination. The border rows fulfilled their purpose of serving as a pollen sink to significantly reduce the amount of pollen dissemination from the test plot. At this location in Mississippi, the distance surrounding a transgenic cotton field test could be reduced and still provide reasonable pollen containment.
Document Type: Article
Language: English
Reprint Address: UMBECK, PF (reprint author), AGRACETUS INC, MIDDLETON, WI 53562 USA
Addresses:
1. UNIV WISCONSIN, DEPT STAT, MADISON, WI 53706 USA
2. USDA ARS, CROP SCI RES LAB, MISSISSIPPI STATE, MS 39762 USA
Publisher: ENTOMOL SOC AMER, 9301 ANNAPOLIS RD, LANHAM, MD 20706
Subject Category: Entomology
IDS Number: GW112
ISSN: 0022-0493
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