| | |  | | | | Record from Web of Science® | | | | | | |
| INVITRO DEVELOPMENT OF MURINE T-CELLS FROM PRETHYMIC AND PRELIVER EMBRYONIC YOLK-SAC HEMATOPOIETIC STEM-CELLS |
|
|
| Author(s): LIU CP, AUERBACH R |
| Source: DEVELOPMENT Volume: 113 Issue: 4 Pages: 1315-1323 Published: DEC 1991 |
| Times Cited: 62 References: 34 |
| Abstract: Mature T cells are derived from prethymic stem cells, which arise at one or more extrathymic sites and enter and differentiate in the thymus. The nature of these prethymic stem cells is a critical factor for the formation of the T-cell repertoire. Although the bone marrow of adult mice can provide such stem cells, their origin during murine embryogenesis is still undetermined. Among potential sites for these progenitor cells are the fetal liver and the embryonic yolk sac. Our studies focus on the yolk sac, both because the yolk sac appears earlier than any other proposed site, and because the mammalian yolk sac is the first site of hematopoiesis. Although it has been shown that the yolk sac in midgestation contains stem cells that can enter the thymic rudiment and differentiate toward T-cell lineage, our aim was to analyze the developmental potential of cells in the yolk sac from earlier stages, prior to the formation of the liver and any other internal organ. We show here that the yolk sac from 8- and 9-day embryos (2-9 and 13-19 somites, respectively) can reconstitute alymphoid congenic fetal thymuses and acquire mature T-cell-specific characteristics. Specifically, thymocytes derived from the early embryonic yolk sac can progress to the expression of mature T lymphocyte markers including CD3/T-cell receptor (TCR), CD4 and CD8. In contrast, we have been unable to document the presence of stem cells within the embryo itself at these early stages. These results support the hypothesis that the stem cells capable of populating the thymic rudiment originate in the yolk sac, and that their presence as early as at the 2- to 9-somite stage may indicate that prethymic stem cells found elsewhere in the embryo at later times may have been derived by migration from this extra-embryonic site. Our experimental design does not exclude the possibility of multiple origins of prethymic stem cells of which the yolk sac may provide the first wave of stem cells in addition to other later waves of cells. |
| Document Type: Article |
| Language: English |
| Reprint Address: LIU, CP (reprint author), UNIV WISCONSIN, CTR DEV BIOL, MADISON, WI 53706 USA |
| Publisher: COMPANY OF BIOLOGISTS LTD, BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL |
| Subject Category: Developmental Biology |
| IDS Number: GZ001 |
| ISSN: 0950-1991 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |