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| NEW DNA MARKERS IN THE HUNTINGTONS-DISEASE GENE CANDIDATE REGION |
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| Author(s): LIN CS, ALTHERR M, BATES G, WHALEY WL, READ AP, HARRIS R, LEHRACH H, WASMUTH JJ, GUSELLA JF, MACDONALD ME |
| Source: SOMATIC CELL AND MOLECULAR GENETICS Volume: 17 Issue: 5 Pages: 481-488 Published: SEP 1991 |
| Times Cited: 26 References: 24 |
| Abstract: The search for the Huntington's disease (HD) gene has prompted construction of a complete long-range restriction map of a 2.5-Mb candidate region, distal to the DNA marker D4S10. To facilitate the procurement of cloned DNA from this candidate region, we have augmented the existing regional mapping panel of somatic cell hybrids with hybrid HHW1071 containing a t(4p16;12) chromosome from a patient with Wolf-Hirschhorn syndrome. This translocation maps between D4S180 and D4S127, subdividing the HD candidate region and setting a proximal limit to the Wolf-Hirschhorn syndrome region. Using the expanded mapping panel, we have regionally assigned 14 independently cloned cosmids, five proximal to the t(4;12) breakpoint in the same region as D4S10 and nine distal to the breakpoint. By a combination of overlap with previously mapped cosmids and pulsed-field gel analysis, each of these cosmids has been positioned on the long-range restriction map of 4p16.3, increasing the clone coverage of the candidate region to approximately 40%. Single-copy probes from mapped cosmids were used to identify eight new DNA polymorphisms spanning the HD candidate region. These new DNA markers should prove valuable for analysis of recombination and linkage disequilibrium in HD, as well as for preclinical diagnosis of the disorder. |
| Document Type: Article |
| Language: English |
| Reprint Address: LIN, CS (reprint author), MASSACHUSETTS GEN HOSP, NEUROGENET LAB, BOSTON, MA 02114 USA |
Addresses:
1. HARVARD UNIV, SCH MED, DEPT GENET, BOSTON, MA 02115 USA
2. UNIV CALIF IRVINE, DEPT BIOL CHEM, IRVINE, CA 92717 USA
3. IMPERIAL CANC RES FUND, LONDON WC2A 3PX, ENGLAND
4. ST MARYS HOSP, DEPT MED GENET, MANCHESTER M13 0JH, LANCS ENGLAND |
| Publisher: PLENUM PUBL CORP, 233 SPRING ST, NEW YORK, NY 10013 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity |
| IDS Number: GZ551 |
| ISSN: 0740-7750 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |