| | |  | | | | Record from Web of Science® | | | | | | |
| ISOZYMES OF CYCLIC-3',5'-NUCLEOTIDE PHOSPHODIESTERASES IN RENAL EPITHELIAL LLC-PK1 CELLS |
|
|
| Author(s): RASSIER ME, MCINTYRE SJ, YAMAKI M, TAKEDA S, LIN JT, DOUSA TP |
| Source: KIDNEY INTERNATIONAL Volume: 41 Issue: 1 Pages: 88-99 Published: JAN 1992 |
| Times Cited: 16 References: 46 |
| Abstract: Metabolism of cAMP and cGMP by the major types (families) of cyclic-3',5'-nucleotide phosphodiesterases (PDE) was studied in confluent renal epithelial LLC-PK1 cells grown in vitro. LLC-PK1 cells mainly contain the cAMP-specific rolipram-sensitive PDE type-IV (PDE-IV), the Ca2+-calmodulin dependent PDE type-I and cGMP-specific PDE type-V; all these PDEs are mainly localized in cytosol. Analysis of PDE activities in soluble extract of LLC-PK1 cell homogenate by FPLC ionex chromatography on Mono-Q column also disclosed the presence of low activities of cGMP-stimulated PDE-II and PDE-III. Moreover, activity of PDE-IV was resolved into four distinct chromatographic peaks. The increase of cAMP level in response to incubation of intact LLC-PK1 cells with vasopressin (AVP) was markedly enhanced in the presence of rolipram, but not in the presence of other PDE isozyme-specific inhibitors. Incubation with AVP and atriopeptin (ANP) together resulted in increase in cGMP and a small decrease of cAMP accumulation in LLC-PK1 cells. Results of these studies first show that the LLC-PK1 cells contain all five major types of PDE isozymes where PDE-IV, PDE-I and PDE-V are quantitatively predominant. The rolipram-sensitive PDE-IV, present in several chromatographically distinct forms, appears to be the key PDE isozyme involved in control of cAMP generated in response to stimulation by AVP in LLC-PK1 cells. |
| Document Type: Article |
| Language: English |
Addresses:
1. MAYO CLIN & MAYO FDN, MAYO MED SCH, DIV NEPHROL, NEPHROL RES UNIT, 921B GUGGENHEIM BLDG, ROCHESTER, MN 55905 USA
2. MAYO CLIN & MAYO FDN, MAYO MED SCH, DEPT PHYSIOL & BIOPHYS, ROCHESTER, MN 55905 USA |
| Publisher: BLACKWELL SCIENCE INC, 350 MAIN ST, MALDEN, MA 02148 |
| Subject Category: Urology & Nephrology |
| IDS Number: GX918 |
| ISSN: 0085-2538 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |