| OPTICAL ANALYSIS OF SYNAPTIC VESICLE RECYCLING AT THE FROG NEUROMUSCULAR-JUNCTION |
|
|
| Author(s): BETZ WJ, BEWICK GS |
| Source: SCIENCE Volume: 255 Issue: 5041 Pages: 200-203 Published: JAN 10 1992 |
| Times Cited: 448 References: 27 |
| Abstract: The fluorescent dyes FM1-43 and RH414 label motor nerve terminals in an activity-dependent fashion that involves dye uptake by synaptic vesicles that are recycling. This allows optical monitoring of vesicle recycling in living nerve terminals to determine how recycled vesicles reenter the vesicle pool. The results suggest that recycled vesicles mix with the pool morphologically and functionally. One complete cycle of release of transmitter, recycling of a vesicle, and rerelease of transmitter appears to take about 1 minute. |
| Document Type: Article |
| Language: English |
| Reprint Address: BETZ, WJ (reprint author), UNIV COLORADO, SCH MED, DEPT PHYSIOL, DENVER, CO 80262 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: GY704 |
| ISSN: 0036-8075 |