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| DNA-BINDING ACTIVITY OF RECOMBINANT SRY FROM NORMAL MALES AND XY FEMALES |
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| Author(s): HARLEY VR, JACKSON DI, HEXTALL PJ, HAWKINS JR, BERKOVITZ GD, SOCKANATHAN S, LOVELLBADGE R, GOODFELLOW PN |
| Source: SCIENCE Volume: 255 Issue: 5043 Pages: 453-456 Published: JAN 24 1992 |
| Times Cited: 329 References: 44 |
| Abstract: The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence AACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination. |
| Document Type: Article |
| Language: English |
Addresses:
1. IMPERIAL CANC RES FUND, HUMAN MOLEC GENET LAB, LINCOLNS INN FIELDS, LONDON WC2A 3PX, ENGLAND
2. JOHNS HOPKINS CHILDREN CTR, DIV PEDIAT ENDOCRINOL, BALTIMORE, MD 21205 USA
3. NATL INST MED RES, MRC, EUKARYOT MOLEC GENET LAB, LONDON NW7 1AA, ENGLAND |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: HA590 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |