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| MULTIPLE MITOCHONDRIAL-DNA DELETIONS IN AN ELDERLY HUMAN INDIVIDUAL |
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| Author(s): ZHANG C, BAUMER A, MAXWELL RJ, LINNANE AW, NAGLEY P |
| Source: FEBS LETTERS Volume: 297 Issue: 1-2 Pages: 34-38 Published: FEB 3 1992 |
| Times Cited: 165 References: 21 |
| Abstract: We have used the polymerase chain reaction (PCR) to study deletions in the mitochondrial DNA (mtDNA) of an elderly human individual. An extended set of PCR primers has been utilised to identify 10 mitochondrial DNA deletions in a 69-year-old female subject with no known mitochondrial disease. The particular deletions visualised as PCR products depended on the primer pairs used, such that the more distantly separated PCR primers enabled visualisation of larger deletions. Some deletions were common to the heart, brain and skeletal muscle, whereas others were apparently specific to individual tissues. DNA sequencing analysis of PCR products showed that short direct repeat sequences (5 to 13 bp) flanked all deletion breakpoints; in most cases one copy of the repeat was deleted. It is proposed that the accumulation of such multiple deletions is a general phenomenon during the ageing process. |
| Document Type: Article |
| Language: English |
Addresses:
1. MONASH UNIV, DEPT BIOCHEM, CLAYTON, VIC 3168 AUSTRALIA
2. MONASH UNIV, CTR MOLEC BIOL & MED, CLAYTON, VIC 3168 AUSTRALIA |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| Subject Category: Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| IDS Number: HD568 |
| ISSN: 0014-5793 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |