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| CYTOKINE STIMULATION OF MULTILINEAGE HEMATOPOIESIS FROM IMMATURE HUMAN-CELLS ENGRAFTED IN SCID MICE |
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| Author(s): LAPIDOT T, PFLUMIO F, DOEDENS M, MURDOCH B, WILLIAMS DE, DICK JE |
| Source: SCIENCE Volume: 255 Issue: 5048 Pages: 1137-1141 Published: FEB 28 1992 |
| Times Cited: 351 References: 30 |
| Abstract: Severe combined immunodeficient (SCID) mice transplanted with human bone marrow were treated with human mast cell growth factor, a fusion of interleukin-3 and granulocyte-macrophage colony-stimulating factor (PIXY321), or both, starting immediately or 1 month later. Immature human cells repopulated the mouse bone marrow with differentiated human cells of multiple myeloid and lymphoid lineages; inclusion of erythropoietin resulted in human red cells in the peripheral blood. The bone marrow of growth factor-treated mice contained both multipotential and committed myeloid and erythroid progenitors, whereas mice not given growth factors had few human cells and only granulocyte-macrophage progenitors. Thus, this system allows the detection of immature human cells, identification of the growth factors that regulate them, and the establishment of animal models of human hematopoietic diseases. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV TORONTO, HOSP SICK CHILDREN, RES INST, DEPT GENET, TORONTO M5G 1X8, ONTARIO CANADA
2. IMMUNEX CORP, DEPT EXPTL HEMATOL, SEATTLE, WA 98101 USA
3. UNIV TORONTO, DEPT MOLEC & MED GENET, TORONTO M5S 1A1, ONTARIO CANADA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: HF632 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |