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| ROLES OF A 106-BP ORIGIN ENHANCER AND ESCHERICHIA-COLI DNAA PROTEIN IN REPLICATION OF PLASMID R6K |
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| Author(s): WU F, GOLDBERG I, FILUTOWICZ M |
| Source: NUCLEIC ACIDS RESEARCH Volume: 20 Issue: 4 Pages: 811-817 Published: FEB 25 1992 |
| Times Cited: 37 References: 55 |
| Abstract: A dnaA 'null' strain could not support replication of intact plasmid R6K or derivatives containing combinations of its three replication origins (alpha, gamma, beta). DnaA binds in vitro to sites in two functionally distinct segments of the central gamma-origin. The 277-bp core segment is common to all three origins and contains DnaA box 2, which cannot be deleted without preventing replication. Immediately to the left of the core lies the 106-bp origin enhancer, which contains DnaA box 1. When the origin enhancer is deleted, the core alone can still initiate replication if levels of wt pi-protein are decreased or if copy-up pi-mutant proteins are provided in trans. DnaA does not effect expression of R6K replication initiator protein-pi, although several DnaA boxes were identified in the coding segment of the pir gene, which encodes pi. Together these data suggest that a single DnaA box, 2, is sufficient for initiation from the gamma-origin and might be sufficient and required for the activity of the alpha and beta-origins as well. Implications of the DnaA protein binding to two domains of the gamma-origin and the role of the 106-bp origin enhancer in replication are discussed. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV WISCONSIN, DEPT BACTERIOL, 1550 LINDEN DR, MADISON, WI 53706 USA |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: HG350 |
| ISSN: 0305-1048 |
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