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| ASSESSMENT OF PROTEIN MODELS WITH 3-DIMENSIONAL PROFILES |
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| Author(s): LUTHY R, BOWIE JU, EISENBERG D |
| Source: NATURE Volume: 356 Issue: 6364 Pages: 83-85 Published: MAR 5 1992 |
| Times Cited: 943 References: 30 |
| Abstract: As methods for determining protein three-dimensional (3D) structure develop, a continuing problem is how to verify that the final protein model is correct. The revision of several protein models to correct errors 1-6 has prompted the development of new criteria for judging the validity of X-ray 7-9 and NMR 10,11 structures, as well as the formation of energetic 12-14 and empirical methods 15,16 to evaluate the correctness of protein models. The challenge is to distinguish between a mistraced or wrongly folded model, and one that is basically correct, but not adequately refined. We show that an effective test of the accuracy of a 3D protein model is a comparison of the model to its own amino-acid sequence, using a 3D profile 16, computed from the atomic coordinates of the structure 3D profiles of correct protein structures match their own sequences with high scores. In contrast, 3D profiles for protein models known to be wrong score poorly. An incorrectly modelled segment in an otherwise correct structure can be identified by examining the profile score in a moving-window scan. The accuracy of a protein model can be assessed by its 3D profile, regardless of whether the model has been derived by X-ray, NMR or computational procedures. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV CALIF LOS ANGELES, INST MOLEC BIOL, LOS ANGELES, CA 90024 USA
2. UNIV CALIF LOS ANGELES, DEPT CHEM & BIOCHEM, LOS ANGELES, CA 90024 USA |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: HG602 |
| ISSN: 0028-0836 |
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