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| CHARACTERIZATION OF PEPTIDES BOUND TO THE CLASS-I MHC MOLECULE HLA-A2.1 BY MASS-SPECTROMETRY |
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| Author(s): HUNT DF, HENDERSON RA, SHABANOWITZ J, SAKAGUCHI K, MICHEL H, SEVILIR N, COX AL, APPELLA E, ENGELHARD VH |
| Source: SCIENCE Volume: 255 Issue: 5049 Pages: 1261-1263 Published: MAR 6 1992 |
| Times Cited: 864 References: 30 |
| Abstract: Antigens recognized by T cells are expressed as peptides bound to major histocompatibility complex (MHC) molecules. Microcapillary high-performance liquid chromatography - electrospray ionization-tandem mass spectrometry was used to fractionate and sequence subpicomolar amounts of peptides isolated from the MHC molecule HLA-A2.1. Of 200 different species quantitated, eight were sequenced and four were found in cellular proteins. All were nine residues long and shared a distinct structural motif. The sensitivity and speed of this approach should enhance the analysis of peptides from small quantities of virally infected and transformed cells as well as those associated with autoimmune disease states. |
| Document Type: Article |
| Language: English |
| Reprint Address: HUNT, DF (reprint author), UNIV VIRGINIA, DEPT CHEM, CHARLOTTESVILLE, VA 22903 USA |
Addresses:
1. UNIV VIRGINIA, SCH MED, DEPT MICROBIOL, CHARLOTTESVILLE, VA 22908 USA
2. UNIV VIRGINIA, SCH MED, BEIRNE CARTER CTR IMMUNOL RES, CHARLOTTESVILLE, VA 22908 USA
3. NCI, CELL BIOL LAB, BETHESDA, MD 20892 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: HG677 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |