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| THE DISTRIBUTION OF 13-GABA-A RECEPTOR SUBUNIT MESSENGER-RNAS IN THE RAT-BRAIN .1. TELENCEPHALON, DIENCEPHALON, MESENCEPHALON |
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| Author(s): WISDEN W, LAURIE DJ, MONYER H, SEEBURG PH |
| Source: JOURNAL OF NEUROSCIENCE Volume: 12 Issue: 3 Pages: 1040-1062 Published: MAR 1992 |
| Times Cited: 1,108 References: 88 |
| Abstract: The expression patterns of 13 GABA(A) receptor subunit encoding genes (alpha(1)-alpha(6), beta(1)-beta(3), gamma(1)-gamma(3), delta) were determined in adult rat brain by in situ hybridization. Each mRNA displayed a unique distribution, ranging from ubiquitous (alpha(1) mRNA) to narrowly confined (alpha(6) mRNA was present only in cerebellar granule cells). Some neuronal populations coexpressed large numbers of subunit mRNAs, whereas in others only a few GABA(A) receptor-specific mRNAs were found. Neocortex, hippocampus, and caudate-putamen displayed complex expression patterns, and these areas probably contain a large diversity of GABA(A) receptors. In many areas, a consistent coexpression was observed for alpha(1) and beta(2) mRNAs, which often colocalized with gamma(2) mRNA. The alpha(1)beta(2) combination was abundant in olfactory bulb, globus pallidus, inferior colliculus, substantia nigra pars reticulata, globus pallidus, zona incerta, subthalamic nucleus, medial septum, and cerebellum. Colocalization was also apparent for the alpha(2) and beta(3) mRNAs, and these predominated in areas such as amygdala and hypothalamus. The alpha(3) mRNA occurred in layers V and VI of neocortex and in the reticular thalamic nucleus. In much of the forebrain, with the exception of hippocampal pyramidal cells, the alpha(4) and delta-transcripts appeared to codistribute. In thalamic nuclei, the only abundant GABA(A) receptor mRNAs were those of alpha(1), alpha(4), beta(2), and delta. In the medial geniculate thalamic nucleus, alpha(1), alpha(4), beta(2), delta, and gamma(3) mRNAs were the principal GABA(A) receptor transcripts. The alpha(5) and beta(1) mRNAs generally colocalized and may encode predominantly hippocampal forms of the GABA(A) receptor. These anatomical observations support the hypothesis that alpha(1)beta(2)gamma(2) receptors are responsible for benzodiazepine I (BZ I) binding, whereas receptors containing alpha(2), alpha(3), and alpha(5) contribute to subtypes of the BZ II site. Based on significant mismatches between alpha(4)/delta and gamma mRNAs, we suggest that in vivo, the alpha(4) subunit contributes to GABA(A) receptors that lack BZ modulation. |
| Document Type: Article |
| Language: English |
| Reprint Address: WISDEN, W (reprint author), UNIV HEIDELBERG, ZENTRUM MOLEK BIOL, MOLEC NEUROENDOCRINOL LAB, NEUENHEIMER FELD 282, W-6900 HEIDELBERG, GERMANY |
| Publisher: SOC NEUROSCIENCE, 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 |
| Subject Category: Neurosciences |
| IDS Number: HJ353 |
| ISSN: 0270-6474 |
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