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STATUS OF THE ICP34.5-GENE IN HERPES-SIMPLEX VIRUS TYPE-1 STRAIN-17
Author(s): DOLAN A, MCKIE E, MACLEAN AR, MCGEOCH DJ
Source: JOURNAL OF GENERAL VIROLOGY    Volume: 73    Pages: 971-973    Part: Part 4    Published: APR 1992  
Times Cited: 33     References: 14     
Abstract: In the published DNA sequence of herpes simplex virus type 1 (HSV-1) strain 17 the coding region for the neurovirulence factor ICP34.5 is disrupted by a 2 bp insertion relative to the corresponding sequence in HSV-1 strain F; this difference would render 60% of the ICP34.5 coding sequence out of frame in strain 17. Re-investigation of the strain 17 sequence showed that the plasmid clone used as the major sequencing substrate for the region was atypical in that other clones did not possess the 2 bp insertion. It was concluded that the coding sequence for ICP34.5 is intact in HSV-1 strain 17 and that the HSV-1 DNA sequence should be revised at this locus.
Document Type: Article
Language: English
Addresses:
1. UNIV GLASGOW, INST VIROL, MRC, VIROL UNIT, CHURCH ST, GLASGOW G11 5JR, SCOTLAND
Publisher: SOC GENERAL MICROBIOLOGY, HARVEST HOUSE 62 LONDON ROAD, READING, BERKS, ENGLAND RG1 5AS
Subject Category: Biotechnology & Applied Microbiology; Virology
IDS Number: HM861
ISSN: 0022-1317
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