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| CLONING AND CHARACTERIZATION OF INDUCIBLE NITRIC-OXIDE SYNTHASE FROM MOUSE MACROPHAGES |
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| Author(s): XIE QW, CHO HJ, CALAYCAY J, MUMFORD RA, SWIDEREK KM, LEE TD, DING AH, TROSO T, NATHAN C |
| Source: SCIENCE Volume: 256 Issue: 5054 Pages: 225-228 Published: APR 10 1992 |
| Times Cited: 1,622 References: 22 |
| Abstract: Nitric oxide (NO) conveys a variety of messages between cells, including signals for vasorelaxation, neurotransmission, and cytotoxicity. In some endothelial cells and neurons, a constitutive NO synthase is activated transiently by agonists that elevate intracellular calcium concentrations and promote the binding of calmodulin. In contrast, in macrophages, NO synthase activity appears slowly after exposure of the cells to cytokines and bacterial products, is sustained, and functions independently of calcium and calmodulin. A monospecific antibody was used to clone complementary DNA that encoded two isoforms of NO synthase from immunologically activated mouse macrophages. Liquid chromatography-mass spectrometry was used to confirm most of the amino acid sequence. Macrophage NO synthase differs extensively from cerebellar NO synthase. The macrophage enzyme is immunologically induced at the transcriptional level and closely resembles the enzyme in cytokine-treated tumor cells and inflammatory neutrophils. |
| Document Type: Article |
| Language: English |
Addresses:
1. CORNELL UNIV, MED CTR,COLL MED,DEPT MED,DIV HEMATOL ONCOL, BEATRICE & SAMUEL A SEAVER LAB, NEW YORK, NY 10021 USA
2. MERCK SHARP & DOHME LTD, DEPT BIOCHEM & MOLEC BIOL, RAHWAY, NJ 07065 USA
3. CITY HOPE NATL MED CTR, BECKMAN RES INT, DIV IMMUNOL, DUARTE, CA 91010 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: HM896 |
| ISSN: 0036-8075 |
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