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| TRANSFORMATION OF VITIS TISSUE BY DIFFERENT STRAINS OF AGROBACTERIUM-TUMEFACIENS CONTAINING THE T-6B GENE |
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| Author(s): BERRES R, OTTEN L, TINLAND B, MALGARINICLOG E, WALTER B |
| Source: PLANT CELL REPORTS Volume: 11 Issue: 4 Pages: 192-195 Published: MAY 1992 |
| Times Cited: 24 References: 17 |
| Abstract: Stem pieces and leaf disks of Vitis spp. were cocultured with Agrobacterium tumefaciens strains carrying the UidA (beta-glucuronidase = GUS) gene. The transformation efficiency was highly increased by using a modified T-6b gene (a gene from pTiTm4) which interferes with normal growth and allows regeneration of normal Nicotiana rustica plants (Tinland 1990). The strains first tested on stem segments were subsequently tested in a leaf explant system. On leaves the transformation efficiency of the strains was much lower than with stems. Both the T-6b gene and the hsp 70-T-6b gene (a modified T-6b gene under the control of a heat shock promoter) allowed the initiation of GUS-positive buds. |
| Document Type: Article |
| Language: English |
Addresses:
1. INRA, PATHOL VEGETALE LAB, VIGNE & VIN STN, 28 RUE HERRLISHEIM, F-68021 COLMAR, FRANCE
2. INST BIOL MOLEC PLANTES, PHYTOPATHOL MOLEC LAB, F-67084 STRASBOURG, FRANCE |
| Publisher: SPRINGER VERLAG, 175 FIFTH AVE, NEW YORK, NY 10010 |
| Subject Category: Plant Sciences |
| IDS Number: HR344 |
| ISSN: 0721-7714 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |