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| GENE-EXPRESSION AND LOCALIZATION OF OPIOID-PEPTIDES IN IMMUNE CELLS OF INFLAMED TISSUE - FUNCTIONAL-ROLE IN ANTINOCICEPTION |
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| Author(s): PRZEWLOCKI R, HASSAN AHS, LASON W, EPPLEN C, HERZ A, STEIN C |
| Source: NEUROSCIENCE Volume: 48 Issue: 2 Pages: 491-500 Published: MAY 1992 |
| Times Cited: 184 References: 37 |
| Abstract: Our previous studies indicate that endogenous opioids (primarily beta-endorphin) released during stressful stimuli can interact with peripheral opioid receptors to inhibit nociception in inflamed tissue of rats. This study sought to localize opioid precurgor mRNAs and opioid peptides deriving therefrom in inflamed tissue, identify opioid containing cells and demonstrate their functional significance in the inhibition of nociception. In rats with Freund's adjuvant-induced unilateral hindpaw inflammation we show that: (i) pro-opiomelanocortin and proenkephalin-mRNAs (but not prodynorphin mRNA) are abundant in cells of inflamed, but absent in non-inflamed tissue; (ii) numerous cells infiltrating the inflamed subcutaneous tissue are stained intensely with beta-endorphin and [Met]enkephalin (but only few scattered cells with dynorphin) antibodies; (iii) beta-endorphin is present in T- and B-lymphocytes, monocytes and macrophages; and (iv) whole-body irradiation suppresses stress-induced antinociception in the inflamed paw. Taken together, these data suggest that endogenous opioid peptides are synthesized and processed within various types of immune cells at the site of inflammation. Immunosuppression abolishes the intrinsic antinociception in inflammatory tissue confirming the functional significance of these cells.
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| Document Type: Article |
| Language: English |
Addresses:
1. MAX PLANCK INST PSYCHIAT, DEPT NEUROPHARMACOL, W-8033 MARTINSRIED, GERMANY
2. POLISH ACAD SCI, INST PHARMACOL, PL-31343 KRAKOW, POLAND
3. MAX PLANCK INST PSYCHIAT, DEPT NEUROIMMUNOL, W-8033 MARTINSRIED, GERMANY
4. LUDWIG MAXIMILIANS UNIV, DEPT ANESTHESIOL, KLINIKUM GROSSHADERN, W-8000 MUNICH 70, GERMANY |
| Publisher: PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB |
| Subject Category: Neurosciences |
| IDS Number: HT664 |
| ISSN: 0306-4522 |
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