| | |  | | | | Record from Web of Science® | | | | | | |
| VARIANTS OF THE TATA-BINDING PROTEIN CAN DISTINGUISH SUBSETS OF RNA POLYMERASE-I, POLYMERASE-II, AND POLYMERASE-III PROMOTERS |
|
|
| Author(s): SCHULTZ MC, REEDER RH, HAHN S |
| Source: CELL Volume: 69 Issue: 4 Pages: 697-702 Published: MAY 15 1992 |
| Times Cited: 178 References: 30 |
| Abstract: Transcription extracts prepared from yeast that are deficient in the TATA-binding protein (TBP or TFIID) are also impaired in specific promoter recognition by all three nuclear RNA polymerases (pol I, II, and III). Specific initiation can be rescued by the addition of purified recombinant TBP, demonstrating that pol I, II, and III all require this factor, A mutation of TBP has been identified that will function with pol I but not with pol II or III. Conversely, another mutation, which inactivates TATA element binding in vitro, will function with pol I and III promoters but is inactive for a pol II promoter. Thus, it is possible to identify TBP variants that will only function on different subsets of all nuclear promoters. |
| Document Type: Article |
| Language: English |
| Reprint Address: SCHULTZ, MC (reprint author), HUTCHINSON CANC RES CTR, 1124 COLUMBIA ST, SEATTLE, WA 98104 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: HV089 |
| ISSN: 0092-8674 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |