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| SURFACTANT PROTEIN-C IS EXPRESSED IN ALVEOLAR TYPE-II CELLS BUT NOT IN CLARA CELLS OF RAT LUNG |
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| Author(s): KALINA M, MASON RJ, SHANNON JM |
| Source: AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY Volume: 6 Issue: 6 Pages: 594-600 Published: JUN 1992 |
| Times Cited: 93 References: 24 |
| Abstract: The expression of the surfactant-associated proteins in bronchiolar cells remains to be defined. We used in situ hybridization to identify sites of message expression of the surfactant-associated proteins A, B, and C (SP-A, SP-B, and SP-C) in adult and fetal rat lung. The expression of these messages by in situ hybridization was also compared with the localization of SP-A by immunocytochemistry. The localization of SP-A was used to identify type II cells and nonciliated bronchiolar epithelial (Clara) cells in these sections. The cRNA antisense probes for SP-A, SP-B, and SP-C appeared to hybridize over type II cells. Sense probes showed no localization or apparent specific hybridization. Messages for both SP-A and SP-B were also found in nonciliated bronchiolar epithelial (Clara) cells. However, no message for SP-C was observed in these cells. Clara cells from terminal to large bronchioles lacked detectable mRNA for SP-C. Expression of surfactant protein mRNAs was not detectable in type I cells, alveolar macrophages, interstitial cells, or vascular cells. Similarly, in fetal rat lung the messages for SP-A and SP-B but not SP-C were detected in bronchiolar cells. We conclude that rat Clara cells do not express SP-C mRNA, and thus SP-C can be regarded as a specific marker for rat type II cells. |
| Document Type: Article |
| Language: English |
Addresses:
1. NATL JEWISH CTR IMMUNOL & RESP MED, DEPT MED, 1400 JACKSON ST, DENVER, CO 80206 USA
2. UNIV COLORADO, HLTH SCI CTR, DENVER, CO 80262 USA |
| Publisher: AMER LUNG ASSOC, 1740 BROADWAY, NEW YORK, NY 10019 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology; Respiratory System |
| IDS Number: HY984 |
| ISSN: 1044-1549 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |