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| RAT GENE-MAPPING USING PCR-ANALYZED MICROSATELLITES |
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| Author(s): SERIKAWA T, KURAMOTO T, HILBERT P, MORI M, YAMADA J, DUBAY CJ, LINDPAINTER K, GANTEN D, GUENET JL, LATHROP GM, BECKMANN JS |
| Source: GENETICS Volume: 131 Issue: 3 Pages: 701-721 Published: JUL 1992 |
| Times Cited: 308 References: 81 |
| Abstract: One hundred and seventy-four rat loci which contain short tandem repeat sequences were extracted from the GenBank or EMBL data bases and used to define primers for amplification by the polymerase chain reaction (PCR) of the microsatellite regions, creating PCR-formatted sequence-tagged microsatellite sites (STMSs). One hundred and thirty-four STMSs for 118 loci, including 6 randomly cloned STMSs, were characterized: (i) PCR-analyzed loci were assigned to specific chromosomes using a panel of rat x mouse somatic cell hybrid clones. (ii) Length variation of the STMSs among 8 inbred rat strains could be visualized at 85 of 107 loci examined (79.4%). (iii) A genetic map, integrating biochemical, coat color, mutant and restriction fragment length polymorphism loci, was constructed based on the segregation of 125 polymorphic markers in seven rat backcrosses and in two F2 crosses. Twenty four linkage groups were identified, all of which were assigned to a defined chromosome. As a reflection of the bias for coding sequences in the public data bases, the STMSs described herein are often associated with genes. Hence, the genetic map we report coincides with a gene map. The corresponding map locations of the homologous mouse and human genes are also listed for comparative mapping purposes. |
| Document Type: Article |
| Language: English |
| Reprint Address: SERIKAWA, T (reprint author), KYOTO UNIV, FAC MED, INST LAB ANIM, KYOTO 606, JAPAN |
Addresses:
1. CTR ETUD POLYMORPHISME HUMAIN, F-75010 PARIS, FRANCE
2. UNIV HEIDELBERG, DEPT PHARMACOL, W-6900 HEIDELBERG, GERMANY
3. UNIV HEIDELBERG, GERMAN INST HIGH BLOOD PRESSURE, W-6900 HEIDELBERG, GERMANY
4. INST PASTEUR, F-75724 PARIS 15, FRANCE
5. INST RECH INTERDISCIPLINAIRE BIOL HUM, B-1070 BRUSSELS, BELGIUM |
| Publisher: GENETICS, 428 EAST PRESTON ST, BALTIMORE, MD 21202 |
| Subject Category: Genetics & Heredity |
| IDS Number: HZ815 |
| ISSN: 0016-6731 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |