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| A SURVEY OF EXPRESSED GENES IN CAENORHABDITIS-ELEGANS |
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| Author(s): WATERSTON R, MARTIN C, CRAXTON M, HUYNH C, COULSON A, HILLIER L, DURBIN R, GREEN P, SHOWNKEEN R, HALLORAN N, METZSTEIN M, HAWKINS T, WILSON R, BERKS M, DU Z, THOMAS K, THIERRYMIEG J, SULSTON J |
| Source: NATURE GENETICS Volume: 1 Issue: 2 Pages: 114-123 Published: MAY 1992 |
| Times Cited: 339 References: 32 |
| Abstract: As an adjunct to the genomic sequencing of Caenorhabditis elegans, we have investigated a representative cDNA library of 1,517 clones. A single sequence read has been obtained from the 5' end of each clone, allowing its characterization with respect to the public databases, and the clones are being localized on the genome map. The result is the identification of about 1,200 of the estimated 15,000 genes of C. elegans. More than 30% of the inferred protein sequences have significant similarity to existing sequences in the databases, providing a route towards in vivo analysis of known genes in the nematode. These clones also provide material for assessing the accuracy of predicted exons and splicing patterns and will lead to a more accurate estimate of the total number of genes in the organism than has hitherto been available. |
| Document Type: Article |
| Language: English |
| Reprint Address: WATERSTON, R (reprint author), WASHINGTON UNIV, SCH MED, DEPT GENET, BOX 8232, 4566 SCOTT AVE, ST LOUIS, MO 63110 USA |
Addresses:
1. LAWRENCE LIVERMORE NATL LAB, CTR HUMAN GENOME, LIVERMORE, CA 94550 USA
2. MRC, MOLEC BIOL LAB, CAMBRIDGE CB2 2QH, ENGLAND
3. CTR RECH BIOL MOLEC & PHYS MATH, CNRS, F-34044 MONTPELLIER, FRANCE |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Genetics & Heredity |
| IDS Number: JC980 |
| ISSN: 1061-4036 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |