| | |  | | | | Record from Web of Science® | | | | | | |
| GENERATION OF REGION-SPECIFIC AND SPECIES-SPECIFIC EXPRESSED GENE PROBES FROM SOMATIC-CELL HYBRIDS |
|
|
| Author(s): JONES KW, CHEVRETTE M, SHAPERO MH, FOURNIER REK |
| Source: NATURE GENETICS Volume: 1 Issue: 4 Pages: 278-283 Published: JUL 1992 |
| Times Cited: 11 References: 44 |
| Abstract: Human genes expressed in interspecific somatic cell hybrids can be cloned specifically by subtractive cDNA hybridization. This approach is based on the observation that cDNA fragments from noncoding segments of mature human transcripts do not form stable heteroduplexes with their rodent homologues under high-stringency hybridization conditions. Thus, small, oligo-dT primed cDNAs from a rat/human hybrid retaining a fragment of human chromosome 17 were enriched for human sequences by hybridization with RNA from a sister clone containing a smaller human chromosome fragment. The enriched probe was used to screen a human cDNA library, and nine expressed genes from within the non-overlap region were obtained. This method should be useful for cloning active human genes from defined chromosome segments. |
| Document Type: Article |
| Language: English |
| Reprint Address: JONES, KW (reprint author), FRED HUTCHINSON CANC RES CTR, DEPT MOLEC MED, SEATTLE, WA 98104 USA |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Genetics & Heredity |
| IDS Number: JC984 |
| ISSN: 1061-4036 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |