| DEVELOPMENT OF THE PARTICLE INFLOW GUN FOR DNA DELIVERY TO PLANT-CELLS |
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| Author(s): FINER JJ, VAIN P, JONES MW, MCMULLEN MD |
| Source: PLANT CELL REPORTS Volume: 11 Issue: 7 Pages: 323-328 Published: JUL 1992 |
| Times Cited: 259 References: 19 |
| Abstract: A simple and inexpensive particle bombardment device was constructed for delivery of DNA to plant cells. The Particle Inflow Gun (PIG) is based on acceleration of DNA-coated tungsten particles using pressurized helium in combination with a partial vacuum. The particles are accelerated directly in a helium stream rather than being supported by a macrocarrier. Bombardment parameters were partially optimized using transient expression assays of a beta-glucuronidase gene in maize embryogenic suspension culture and cowpea leaf tissues. High levels of transient expression of the beta-glucuronidase gene were obtained following bombardment of embryogenic suspension cultures of com and soybean, and leaf tissue of cowpea. Stable transformation of embryogenic tissue of soybean has also been obtained using this bombardment apparatus. |
| Document Type: Article |
| Language: English |
| Reprint Address: FINER, JJ (reprint author), OHIO STATE UNIV, OHIO AGR RES & DEV CTR, DEPT AGRON, WOOSTER, OH 44691 USA |
| Publisher: SPRINGER VERLAG, 175 FIFTH AVE, NEW YORK, NY 10010 |
| Subject Category: Plant Sciences |
| IDS Number: JD358 |
| ISSN: 0721-7714 |