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| EXPRESSION CLONING OF A CDNA-ENCODING A RETINOBLASTOMA-BINDING PROTEIN WITH E2F-LIKE PROPERTIES |
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| Author(s): KAELIN WG, KREK W, SELLERS WR, DECAPRIO JA, AJCHENBAUM F, FUCHS CS, CHITTENDEN T, LI Y, FARNHAM PJ, BLANAR MA, LIVINGSTON DM, FLEMINGTON EK |
| Source: CELL Volume: 70 Issue: 2 Pages: 351-364 Published: JUL 24 1992 |
| Times Cited: 785 References: 73 |
| Abstract: An expression vector was modified to permit the rapid synthesis of purified, P-32-labeled, glutathione S-transferase (GST)-retinoblastoma (RB) fusion proteins. The products were used to screen lambda-gt11 expression libraries, from which we cloned a cDNA encoding a polypeptide (RBAP-1) capable of binding directly to a putative functional domain (the pocket) of the retinoblastoma gene product (RB). The RB "pocket" is known to bind, directly or indirectly, to the cellular transcription factor, E2F, implicated in cell growth control. We have found that RBAP-1 copurifies with E2F, interacts specifically with the adenovirus E4 ORF 6/7 protein, binds specifically and directly to a known E2F DNA recognition sequence, and contains a functional transactivation domain. Therefore, RBAP-1 is a species of E2F and can bind specifically to the RB pocket. |
| Document Type: Article |
| Language: English |
| Reprint Address: KAELIN, WG (reprint author), HARVARD UNIV, SCH MED, DANA FARBER CANC INST, BOSTON, MA 02115 USA |
Addresses:
1. HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA
2. UNIV WISCONSIN, MCARDLE LAB CANC RES, MADISON, WI 53706 USA
3. UNIV CALIF SAN FRANCISCO, SCH MED, HORMONE RES INST, SAN FRANCISCO, CA 94143 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: JE757 |
| ISSN: 0092-8674 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |