| | |  | | | | Record from Web of Science® | | | | | | |
| DIRECTED EVOLUTION OF AN RNA ENZYME |
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| Author(s): BEAUDRY AA, JOYCE GF |
| Source: SCIENCE Volume: 257 Issue: 5070 Pages: 635-641 Published: JUL 31 1992 |
| Times Cited: 270 References: 75 |
| Abstract: An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic function. A population of 10(13) variants of the, Tetrahynena ribozyme, a group I ribozyme that catalyzes sequence-specific cleavage of RNA via a phosphoester transfer mechanism, was generated. This enzyme has a limited ability to cleave DNA under conditions of high temperature or high MgCl2 concentration, or both. A selection constraint was imposed on the population of ribozyme variants such that only those individuals that carried out DNA cleavage under physiologic conditions were amplified to produce "progeny" ribozymes. Mutations were introduced during amplification to maintain heterogeneity in the population. This process was repeated for ten successive generations, resulting in enhanced (100 times) DNA cleavage activity. |
| Document Type: Article |
| Language: English |
| Reprint Address: BEAUDRY, AA (reprint author), SCRIPPS RES INST, DEPT CHEM, LA JOLLA, CA 92037 USA |
Addresses:
1. SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: JF852 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |