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| MOLECULAR-CLONING AND CHARACTERIZATION OF HUMAN ENDOTHELIAL NITRIC-OXIDE SYNTHASE |
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| Author(s): MARSDEN PA, SCHAPPERT KT, CHEN HS, FLOWERS M, SUNDELL CL, WILCOX JN, LAMAS S, MICHEL T |
| Source: FEBS LETTERS Volume: 307 Issue: 3 Pages: 287-293 Published: AUG 3 1992 |
| Times Cited: 363 References: 22 |
| Abstract: The constitutive calcium/calmodulin-dependent nitric oxide (NO) synthase expressed in vascular endothelium shares common biochemical and pharmacologic properties with neuronal NO synthase. However, recent cloning and molecular characterization of NO synthase from bovine endothelial cells indicated the existence of a family of constitutive NO synthases. Accordingly, we undertook molecular cloning and sequence analysis of human endothelial NO synthase. Complementary DNA clones predict a protein of 1,203 amino acids sharing 94% identity with the bovine endothelial protein, but only 60% identity with the rat brain NO synthase isoform. Northern blot analysis with an endothelial-derived cDNA identified a 4.6-4.8 kb mRNA transcript in HUVEC and in situ hybridization localized transcripts to vascular endothelium but not neuronal tissue. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV TORONTO, ST MICHAELS HOSP, DEPT MED, TORONTO M5B 1W8, ONTARIO CANADA
2. UNIV TORONTO, ST MICHAELS HOSP, DIV RENAL, TORONTO M5B 1W8, ONTARIO CANADA
3. UNIV TORONTO, HOSP SICK CHILDREN, DEPT GENET, TORONTO M5G 1X8, ONTARIO CANADA
4. EMORY UNIV, DEPT MED, ATLANTA, GA 30322 USA
5. HARVARD UNIV, BRIGHAM & WOMENS HOSP, SCH MED, DIV CARDIOVASC, BOSTON, MA 02115 USA |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| Subject Category: Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| IDS Number: JH396 |
| ISSN: 0014-5793 |
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