| | |  | | | | Record from Web of Science® | | | | | | |
| SEQUENTIAL TUBULAR CELL AND BASEMENT-MEMBRANE CHANGES IN POLYCYSTIC KIDNEY-DISEASE |
|
|
| Author(s): CARONE FA, NAKAMURA S, PUNYARIT P, KANWAR YS, NELSON WJ |
| Source: JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY Volume: 3 Issue: 2 Pages: 244-253 Published: AUG 1992 |
| Times Cited: 41 References: 33 |
| Abstract: Tubular basement membrane (BM) changes (dysmorphogenesis), cell proliferation, and fluid accumulation related to the altered location of Na,K-ATPase are purported essential key events in the development and progression of renal cysts. These changes were assessed daily in Phenol II (2-amino-4-hydroxyphenyl-5-phenyl thiazole)-treated rats, which rapidly develop marked and progressive cystic change of all collecting tubules (CT). At Day 1, 12% of CT were cystic and their BM were thickened severalfold. At Day 4, 30% of CT were cystic and their BM remained thickened. BM of cystic tubules showed decreased staining for heparan sulfate proteoglycan and increased staining for fibronectin. Proliferation, as determined by (H-3)thymidine, incorporation, was not significant until Day 2 and involved cystic and noncystic tubular cells as well as interstitial cells. As cystic changes progressed, cell proliferation decreased. By immunohistochemistry, the altered location of Na,K-ATPase in epithelial cells lining cysts was primarily detected after Day 2 and consisted of focal loss from basal and/or lateral cell membranes and localization in the cell cytoplasm. Only rarely was Na,K-ATPase localized to the apicol cell membrane. After the removal of Phenol II, cystic tubular cells, BM, and Na,K-ATPase returned to normal. Thus, in this model of polycystic kidney disease, initial cyst formation occurred in tandem with BM structural change whereas cell proliferation and altered location of Na,K-ATPase occurred after the appearance of cysts. |
| Document Type: Article |
| Language: English |
| Reprint Address: CARONE, FA (reprint author), NORTHWESTERN UNIV, SCH MED, DEPT PATHOL, 303 E CHICAGO AVE, CHICAGO, IL 60611 USA |
Addresses:
1. STANFORD UNIV, MED CTR, SCH MED, DEPT MOLEC & CELLULAR PHYSIOL, STANFORD, CA 94305 USA |
| Publisher: WILLIAMS & WILKINS, 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 |
| Subject Category: Urology & Nephrology |
| IDS Number: JK694 |
| ISSN: 1046-6673 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |