| | |  | | | | Record from Web of Science® | | | | | | |
| MAPPING OF NEURAL NITRIC-OXIDE SYNTHASE IN THE RAT SUGGESTS FREQUENT COLOCALIZATION WITH NADPH DIAPHORASE BUT NOT WITH SOLUBLE GUANYLYL CYCLASE, AND NOVEL PARANEURAL FUNCTIONS FOR NITRINERGIC SIGNAL TRANSDUCTION |
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| Author(s): SCHMIDT HHHW, GAGNE GD, NAKANE M, POLLOCK JS, MILLER MF, MURAD F |
| Source: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY Volume: 40 Issue: 10 Pages: 1439-1456 Published: OCT 1992 |
| Times Cited: 573 References: 100 |
| Abstract: Nitric oxide synthases (NOS Types I-III) generate nitric oxide (NO), which in turn activates soluble guanylyl cyclase (GC-S). The distribution of this NO-mediated (nitrinergic) signal transduction pathway in the body is unclear. A polyclonal monospecific antibody to rat cerebellum NOS-I and a monoclonal antibody to rat lung, GC-S were employed to localize the protein components of this pathway in different rat organs and tissues. We confirmed the localization of NOS-I in neurons of the central and peripheral nervous system, where NO may regulate cerebral blood flow and mediate long-term potentiation. GC-S was located in NOS-negative neurons, indicating that NO acts as an intercellular signal molecule or neurotransmitter. However, NOS-I was not confined to neurons but was widely distributed over several non-neural cell types and tissues. These included glia cells, macula densa of kidney, epithelial cells of lung, uterus, and stomach, and islets of Langerhans. Our findings suggest that NOS-I is the most widely distributed isoform of NOS and, in addition to its neural functions, regulates secretion and non-vascular smooth muscle function. With the exception of bone tissue, NADPH-diaphorase (NADPH-d) activity was generally co-localized with NOS-I immunoreactivity in both neural and non-neural cells, and is a suitable histochemical marker for NOS-I but not a selective neuronal marker. |
| Document Type: Note |
| Language: English |
Addresses:
1. NORTHWESTERN UNIV, SCH MED, DEPT PHARMACOL, CHICAGO, IL 60611 USA
2. ABBOTT LABS, DEPT CELLULAR & MICROSCOP RES, N CHICAGO, IL 60064 USA
3. VASC BIOL GRP, ABBOTT PK, IL 60064 USA |
| Publisher: HISTOCHEMICAL SOC INC, MT SINAI MEDICAL CENTER 19 EAST 98TH ST SUTIE 9G, NEW YORK, NY 10029 |
| Subject Category: Cell Biology |
| IDS Number: JP519 |
| ISSN: 0022-1554 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |