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| CHARACTERIZATION OF CHROMOSOME-11 TRANSLOCATION BREAKPOINTS AT THE BCL-1 AND PRAD1 LOCI IN CENTROCYTIC LYMPHOMA |
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| Author(s): WILLIAMS ME, SWERDLOW SH, ROSENBERG CL, ARNOLD A |
| Source: CANCER RESEARCH Volume: 52 Issue: 19 Pages: S5541-S5544 Supplement: Suppl. S Published: OCT 1 1992 |
| Times Cited: 109 References: 53 |
| Abstract: The chromosome 11q13 bcl-1 locus is rearranged in the majority of centrocytic lymphomas, a CD5-positive B-cell non-Hodgkins lymphoma, as a result of reciprocal translocation with the 14q32 immunoglobulin heavy chain genes. Although several 11q13 bcl-1 breakpoint sites have been characterized, a postulated bcl-1 oncogene was not identified. Recently, however, a gene encoding cyclin D1, designated PRAD1, was proposed as a candidate bcl-1 oncogene; accumulated evidence now indicates this gene is bcl-1. To further characterize 11q13 breakpoints in B-cell neoplasms, we analyzed 26 centrocytic lymphomas and 68 other B-cell cancers by Southern blot using a panel of breakpoint probes spanning 110 kilobases of the bcl-1 and PRAD1 loci. Nineteen centrocytic cases (73%) showed rearrangement, 15 at bcl-1 breakpoint sites and 5 at PRAD1 sites. One case was rearranged at both bcl-1 and PRAD1 loci. All but the latter case showed comigration of rearranged bcl-1 or PRAD1 bands and immunoglobulin heavy chain joining gene bands, consistent with the t(11;14). bcl-1 rearrangement was present in only one of 68 noncentrocytic B-cell neoplasms; none showed PRAD1 rearrangement. Thus, bcl-1 and PRAD1 rearrangement is strongly associated with centrocytic lymphoma, providing a useful molecular marker for classifying this subtype of lymphoma and suggesting an important role for PRAD1 cyclin D1 in the pathogenesis of this neoplasm. |
| Document Type: Proceedings Paper |
| Language: English |
Addresses:
1. UNIV VIRGINIA, SCH MED, DEPT INTERNAL MED, CHARLOTTESVILLE, VA 22908 USA
2. UNIV VIRGINIA, SCH MED, DEPT PATHOL, CHARLOTTESVILLE, VA 22908 USA
3. UNIV CINCINNATI, DEPT PATHOL & LAB MED, CINCINNATI, OH 45267 USA
4. MASSACHUSETTS GEN HOSP, ENDOCRINE UNIT, BOSTON, MA 02114 USA |
| Publisher: AMER ASSOC CANCER RESEARCH, PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 |
| Subject Category: Oncology |
| IDS Number: JR138 |
| ISSN: 0008-5472 |
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