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| THE PRECURSOR OF NF-KAPPA-B P50 HAS I-KAPPA-B-LIKE FUNCTIONS |
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| Author(s): RICE NR, MACKICHAN ML, ISRAEL A |
| Source: CELL Volume: 71 Issue: 2 Pages: 243-253 Published: OCT 16 1992 |
| Times Cited: 385 References: 39 |
| Abstract: The C-terminal half of the p105 precursor of the NF-kappaB p50 subunit contains ankyrin-like repeats similar to those in IkappaB molecules, which are known to retain NF-kappaB complexes in the cytoplasm. We demonstrate that in various cell lines p105 is found associated with either c-rel or p65 in the cytoplasm and serves IkappaB-like functions. p105 retains c-rel or p65 in the cytoplasm in cotransfection experiments in COS cells. It also inhibits DNA binding by c-rel in gel retardation assays. Stable interaction of p105 with c-rel or p65 requires the putative dimerization domain in the conserved rel homology region of p105, as well as a second contact with the IkappaB-related C-terminal part of p105. Pulse-chase experiments indicate that cytoplasmic complexes of p105 with c-rel or p65 give rise to cytoplasmic as well as nuclear p50-c-rel and p50-p65, respectively, probably through processing of p105. Thus, p105, like the IkappaBs, controls the subcellular localization and hence the transcriptional activity of at least two other members of the rel/NF-kappaB family. |
| Document Type: Article |
| Language: English |
| Reprint Address: RICE, NR (reprint author), FREDERICK CANC RES & DEV CTR, ABL BASIC RES PROGRAM, MOLEC VIROL & CARCINOGENESIS LAB, FREDERICK, MD 21702 USA |
Addresses:
1. STANFORD UNIV, PROGRAM CANC BIOL, STANFORD, CA 94305 USA
2. INST PASTEUR, UNITE BIOL MOLEC EXPRESS GENIQUE, F-75724 PARIS 15, FRANCE |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: JU395 |
| ISSN: 0092-8674 |
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