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| AGONIST-MODULATED PALMITOYLATION OF BETA-2-ADRENERGIC RECEPTOR IN SF9 CELLS |
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| Author(s): MOUILLAC B, CARON M, BONIN H, DENNIS M, BOUVIER M |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 267 Issue: 30 Pages: 21733-21737 Published: OCT 25 1992 |
| Times Cited: 158 References: 32 |
| Abstract: The palmitoylation of the human beta2-adrenergic receptor (beta2-AR) was studied in recombinant baculo-virus-infected insect Sf9 cells. At 48 h post-infection, a high level expression of an epitope-tagged beta2-AR (10-25 pmol/mg protein) was detected by [I-125]iodocyanopindolol ([I-125]CYP) binding assays. The identity of the receptor was confirmed both by photoaffinity labeling and immunoblotting. The fusion receptor displayed typical beta2-AR pharmacological properties and conferred a beta-adrenergic sensitive adenylyl cyclase activity to the Sf9 cells. Moreover, exposure of the Sf9 cells to the beta-adrenergic agonist isoproterenol induced a rapid desensitization of the receptor-stimulated adenylyl cyclase activity. Purification of the epitope-tagged beta2-AR by immunoprecipitation as well as by alprenolol-Sepharose affinity chromatography revealed that the receptor is covalently modified with palmitic acid in the insect cells as is observed in mammalian cells. In addition, short-term incubation of the cells with isoproterenol led to a specific increase in the incorporation of [H-3]palmitate in the receptor, consistent with a rapid agonist-modulated turnover of the beta2-AR-attached palmitic acid. These results suggest that agonist-mediated regulation of beta2-AR post-translational palmitoylation could represent an other regulatory process for G protein-coupled receptors. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV MONTREAL, DEPT BIOCHEM, POB 6128, STN A, MONTREAL H3C 3J7, QUEBEC CANADA
2. BIOTECHNOL RES INST, MONTREAL H4P 2R2, QUEBEC CANADA |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: JV011 |
| ISSN: 0021-9258 |
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