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| IDENTIFICATION OF PROGRAMMED CELL-DEATH INSITU VIA SPECIFIC LABELING OF NUCLEAR-DNA FRAGMENTATION |
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| Author(s): GAVRIELI Y, SHERMAN Y, BENSASSON SA |
| Source: JOURNAL OF CELL BIOLOGY Volume: 119 Issue: 3 Pages: 493-501 Published: NOV 1992 |
| Times Cited: 7,374 References: 37 |
| Abstract: Programmed cell death (PCD) plays a key role in developmental biology and in maintenance of the steady state in continuously renewing tissues. Currently, its existence is inferred mainly from gel electrophoresis of a pooled DNA extract as PCD was shown to be associated with DNA fragmentation. Based on this observation, we describe here the development of a method for the in situ visualization of PCD at the single-cell level, while preserving tissue architecture. Conventional histological sections, pretreated with protease, were nick end labeled with biotinylated poly dU, introduced by terminal deoxytransferase, and then stained using avidin-conjugated peroxidase. The reaction is specific, only nuclei located at positions where PCD is expected are stained. The initial screening includes: small and large intestine, epidermis, lymphoid tissues, ovary, and other organs. A detailed analysis revealed that the process is initiated at the nuclear periphery, it is relatively short (1-3 h from initiation to cell elimination) and that PCD appears in tissues in clusters. The extent of tissue-PCD revealed by this method is considerably greater than apoptosis detected by nuclear morphology, and thus opens the way for a variety of studies. |
| Document Type: Article |
| Language: English |
| Reprint Address: GAVRIELI, Y (reprint author), HEBREW UNIV JERUSALEM, HADASSAH MED SCH, DEPT EXPTL MED & CANC RES, POB 1172, IL-91010 JERUSALEM, ISRAEL |
Addresses:
1. HEBREW UNIV JERUSALEM, HADASSAH MED SCH, DEPT PATHOL, IL-91010 JERUSALEM, ISRAEL |
| Publisher: ROCKEFELLER UNIV PRESS, 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 |
| Subject Category: Cell Biology |
| IDS Number: JV269 |
| ISSN: 0021-9525 |
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