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| COMPARATIVE GENOMIC HYBRIDIZATION FOR MOLECULAR CYTOGENETIC ANALYSIS OF SOLID TUMORS |
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| Author(s): KALLIONIEMI A, KALLIONIEMI OP, SUDAR D, RUTOVITZ D, GRAY JW, WALDMAN F, PINKEL D |
| Source: SCIENCE Volume: 258 Issue: 5083 Pages: 818-821 Published: OCT 30 1992 |
| Times Cited: 2,074 References: 11 |
| Abstract: Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified. |
| Document Type: Article |
| Language: English |
| Reprint Address: KALLIONIEMI, A (reprint author), UNIV CALIF SAN FRANCISCO, DEPT LAB MED, DIV MOLEC CYTOMETRY, SAN FRANCISCO, CA 94143 USA |
Addresses:
1. WESTERN GEN HOSP, MRC, HUMAN GENET UNIT, EDINBURGH EH4 2XU, MIDLOTHIAN SCOTLAND |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: JV692 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |