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| LARGE-SCALE CDNA SEQUENCING FOR ANALYSIS OF QUANTITATIVE AND QUALITATIVE ASPECTS OF GENE-EXPRESSION |
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| Author(s): OKUBO K, HORI N, MATOBA R, NIIYAMA T, FUKUSHIMA A, KOJIMA Y, MATSUBARA K |
| Source: NATURE GENETICS Volume: 2 Issue: 3 Pages: 173-179 Published: NOV 1992 |
| Times Cited: 414 References: 19 |
| Abstract: Large scale sequencing of cDNAs provides a complementary approach to structural analysis of the human genome by generating expressed sequence tags (ESTs). We have initiated the large-scale sequencing of a 3'-directed cDNA library from the human liver cell line HepG2, that is a non-biased representation of the mRNA population. 982 random cDNA clones were sequenced yielding more than 270 kilobases. A significant portion of the identified genes encoded secretable proteins and components for protein-synthesis. The abundance of cDNA species varied from 2.2% to less than 0.004%. Fifty two percent of the mRNA were abundant species consisting of 173 genes and the rest were non-abundant, consisting of about 6,600 genes. |
| Document Type: Article |
| Language: English |
Addresses:
1. OSAKA UNIV, INST MOLEC & CELLULAR BIOL, 1-3 YAMADA OKA, SUITA, OSAKA 565 JAPAN |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Genetics & Heredity |
| IDS Number: JW165 |
| ISSN: 1061-4036 |
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