| | |  | | | | Record from Web of Science® | | | | | | |
| SINGLE PASS SEQUENCING AND PHYSICAL AND GENETIC-MAPPING OF HUMAN BRAIN CDNAS |
|
|
| Author(s): KHAN AS, WILCOX AS, POLYMEROPOULOS MH, HOPKINS JA, STEVENS TJ, ROBINSON M, ORPANA AK, SIKELA JM |
| Source: NATURE GENETICS Volume: 2 Issue: 3 Pages: 180-185 Published: NOV 1992 |
| Times Cited: 156 References: 25 |
| Abstract: We have performed single pass sequencing of 1,024 human brain cDNAs, over 900 of which seem to represent new human genes. Library prescreening with total brain cDNA significantly reduced repeated sequencing of highly represented cDNAs. A subset of sequenced cDNAs were physically mapped to their chromosomal locations using gene-specific STS primers derived from 3' untranslated regions. We have also determined that human brain cDNAs represent a rich source of gene-associated polymorphic markers. Microsatellite-containing cDNAs can be physically mapped and converted to highly informative genetic markers, thus facilitating integration of the human physical, expression and genetic maps. |
| Document Type: Article |
| Language: English |
| Reprint Address: KHAN, AS (reprint author), UNIV COLORADO, HLTH SCI CTR, DEPT PHARMACOL, DENVER, CO 80262 USA |
Addresses:
1. VET ADM SCHIZOPHRENIA CTR, MOLEC BIOL LAB, DENVER, CO 80220 USA
2. NIMH, BIOCHEM GENET LAB, WASHINGTON, DC 20032 USA |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Genetics & Heredity |
| IDS Number: JW165 |
| ISSN: 1061-4036 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |