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| THE HUMAN XIST GENE - ANALYSIS OF A 17 KB INACTIVE X-SPECIFIC RNA THAT CONTAINS CONSERVED REPEATS AND IS HIGHLY LOCALIZED WITHIN THE NUCLEUS |
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| Author(s): BROWN CJ, HENDRICH BD, RUPERT JL, LAFRENIERE RG, XING Y, LAWRENCE J, WILLARD HF |
| Source: CELL Volume: 71 Issue: 3 Pages: 527-542 Published: OCT 30 1992 |
| Times Cited: 430 References: 71 |
| Abstract: X chromosome inactivation in mammalian females results in the cis-limited transcriptional inactivity of most of the genes on one X chromosome. The XIST gene is unique among X-linked genes in being expressed exclusively from the inactive X chromosome. Human XIST cDNAs containing at least eight exons and totaling 17 kb have been isolated and sequenced within the region on the X chromosome known to contain the X inactivation center. The XIST gene includes several tandem repeats, the most 5' of which are evolutionarily conserved. The gene does not contain any significant conserved ORFs and thus does not appear to encode a protein, suggesting that XIST may function as a structural RNA within the nucleus. Consistent with this, fluorescence in situ hybridization experiments demonstrate localization of XIST RNA within the nucleus to a position indistinguishable from the X inactivation-associated Barr body. |
| Document Type: Article |
| Language: English |
Addresses:
1. STANFORD UNIV, DEPT GENET, STANFORD, CA 94305 USA
2. UNIV MASSACHUSETTS, SCH MED, DEPT CELL BIOL, WORCESTER, MA 01655 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: JW435 |
| ISSN: 0092-8674 |
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