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| THE IMMUNE-RESPONSE TO P53 IN BREAST-CANCER PATIENTS IS DIRECTED AGAINST IMMUNODOMINANT EPITOPES UNRELATED TO THE MUTATIONAL HOT-SPOT |
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| Author(s): SCHLICHTHOLZ B, LEGROS Y, GILLET D, GAILLARD C, MARTY M, LANE D, CALVO F, SOUSSI T |
| Source: CANCER RESEARCH Volume: 52 Issue: 22 Pages: 6380-6384 Published: NOV 15 1992 |
| Times Cited: 259 References: 30 |
| Abstract: Alteration of the p53 gene is the most frequent genetic feature of human cancer and leads to overexpression of the altered protein in the tumor cell nucleus. Two diagnostic procedures are currently available to assess p53 mutations: (a) molecular analysis of the gene sequence; and (b) immunohistochemical analysis of p53 protein accumulation. We now report a third approach, serological analysis. Fifteen % of primary breast cancer patients were found to have circulating antibodies to p53 protein by immunoprecipitation or immunoblotting. We have found a close correlation between the presence of such antibodies and bad prognosis such as high histological grade and the absence of hormone receptors. Furthermore, we found that the B-cell response to p53 protein is induced by two immunodominant regions located at the carboxy and amino termini of the protein, outside the central mutational hot spot region. These findings suggest that serological analysis, combined with molecular and histochemical methods, may be suitable for assessing the state of the p53 gene in cancer patients. |
| Document Type: Note |
| Language: English |
Addresses:
1. HOP ST LOUIS, INST GENET MOLEC, INSERM, U301, 27 RUE JULIETTE DODU, F-75010 PARIS, FRANCE
2. CENS, INGN GENET PROT LAB, F-91191 GIF SUR YVETTE, FRANCE
3. HOP ST LOUIS, INST ONCOL MED, F-75010 PARIS, FRANCE
4. HOP ST LOUIS, PHARMACOL EXPTL LAB, F-75010 PARIS, FRANCE
5. UNIV DUNDEE, CRC LABS, DUNDEE DD1 4HN, SCOTLAND |
| Publisher: AMER ASSOC CANCER RESEARCH, 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA |
| Subject Category: Oncology |
| IDS Number: JX754 |
| ISSN: 0008-5472 |
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