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| THE MULTIDRUG RESISTANCE AND CYSTIC-FIBROSIS GENES HAVE COMPLEMENTARY PATTERNS OF EPITHELIAL EXPRESSION |
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| Author(s): TREZISE AEO, ROMANO PR, GILL DR, HYDE SC, SEPULVEDA FV, BUCHWALD M, HIGGINS CF |
| Source: EMBO JOURNAL Volume: 11 Issue: 12 Pages: 4291-4303 Published: DEC 1992 |
| Times Cited: 156 References: 59 |
| Abstract: The cystic fibrosis gene product, CFTR, and the multidrug resistance P-glycoprotein (encoded by the MDR1 gene) are structurally related proteins and both are associated with epithelial chloride channel activities. We have compared their cell-specific expression in the rat by in situ hybridization. In all tissues examined the two genes were found to have complementary patterns of expression, demonstrating exquisite regulation in both cell-specific and temporal fashions. Additionally, a switch in expression from one gene to the other was observed in certain tissues. For example, expression in the intestine switches from CFTR to MDR1 as the cells migrate across the crypt-villus boundary. A switch from CFTR to MDR1 expression was also observed in the uterine epithelium upon pregnancy. These data suggest that CFTR and P-glycoprotein serve analogous roles in epithelial cells and provide additional evidence that P-glycoprotein has a physiological role in regulating epithelial cell volume. The patterns of expression suggest that the regulation of these two genes is coordinately controlled. |
| Document Type: Article |
| Language: English |
| Reprint Address: TREZISE, AEO (reprint author), UNIV OXFORD, JOHN RADCLIFFE HOSP, INST MOLEC MED, IMPERIAL CANC RES FUND LABS, OXFORD OX3 9DU, ENGLAND |
Addresses:
1. HOSP SICK CHILDREN, RES INST, DEPT GENET, TORONTO M5G 1X8, ONTARIO CANADA
2. AFRC, INST ANIM PHYSIOL & GENET RES, CAMBRIDGE CB2 4AT, ENGLAND |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: JY101 |
| ISSN: 0261-4189 |
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