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| REGULATION OF THE DIFFERENTIATION OF TERATOCARCINOMA CELLS INTO PRIMITIVE ENDODERM BY G-ALPHA-I2 |
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| Author(s): WATKINS DC, JOHNSON GL, MALBON CC |
| Source: SCIENCE Volume: 258 Issue: 5086 Pages: 1373-1375 Published: NOV 20 1992 |
| Times Cited: 95 References: 27 |
| Abstract: The amount of the heterotrimeric G protein subunit Galpha(i2) decreases after the induction of F9 teratocarcinoma cells to become primitive endoderm in the presence of retinoic acid (RA). The reduction of the Galpha(i2) protein in F9 cells by antisense RNA expression was associated with (i) loss of receptor-mediated inhibition of adenylyl cyclase; (ii) decreased cell doubling time; (iii) induction of a primitive, endoderm-like phenotype in the absence of RA; and (iv) production of the differentiation marker tissue-type plasminogen activator. Expression of a constitutively active, mutant Galpha(i2) blocked RA-induced differentiation. These data suggest the involvement of Galpha(i2) in the control of stem cell differentiation and provide insight into the involvement of G proteins in growth regulation. |
| Document Type: Article |
| Language: English |
| Reprint Address: WATKINS, DC (reprint author), SUNY STONY BROOK, HLTH SCI CTR, DIABET & METAB DIS RES PROGRAM, STONY BROOK, NY 11794 USA |
Addresses:
1. SUNY STONY BROOK, HLTH SCI CTR, DEPT PHARMACOL, STONY BROOK, NY 11794 USA
2. NATL JEWISH CTR IMMUNOL, DEPT PEDIAT & BASIC SCI, DENVER, CO 80206 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: JY958 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |