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| A MULTIFUNCTIONAL AQUEOUS CHANNEL FORMED BY CFTR |
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| Author(s): HASEGAWA H, SKACH W, BAKER O, CALAYAG MC, LINGAPPA V, VERKMAN AS |
| Source: SCIENCE Volume: 258 Issue: 5087 Pages: 1477-1479 Published: NOV 27 1992 |
| Times Cited: 114 References: 30 |
| Abstract: The cystic fibrosis gene product (CFTR) is a complex protein that functions as an adenosine 3,5-monophosphate (cAMP)-stimulated ion channel and possibly as a regulator of intracellular processes. In order to determine whether the CFTR molecule contains a functional aqueous pathway, anion, water, and urea transport were measured in Xenopus oocytes expressing CFTR. Cyclic AMP agonists induced a Cl- conductance of 94 microsiemens and an increase in water permeability of 4 x 10(-4) centimeter per second that was inhibited by a Cl- channel blocker and was dependent on anion composition. CFTR has a calculated single channel water conductance of 9 x 10(-13) cubic centimeter per second, suggesting a pore-like aqueous pathway. Oocytes expressing CFTR also showed cAMP-stimulated transport of urea but not the larger solute sucrose. Thus CFTR contains a cAMP-stimulated aqueous pore that can transport anions, water, and small solutes. The results also provide functional evidence for water movement through an ion channel. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV CALIF SAN FRANCISCO, CYST FIBROSIS RES CTR, DEPT MED, SAN FRANCISCO, CA 94143 USA
2. UNIV CALIF SAN FRANCISCO, CYST FIBROSIS RES CTR, DEPT PHYSIOL, SAN FRANCISCO, CA 94143 USA
3. UNIV CALIF SAN FRANCISCO, CARDIOVASC RES INST, SAN FRANCISCO, CA 94143 USA
4. UNIV CALIF SAN FRANCISCO, CANC RES INST, SAN FRANCISCO, CA 94143 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: JZ625 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |