| THE RHIZOBIUM-MELILOTI PMI GENE ENCODES A NEW TYPE OF PHOSPHOMANNOSE ISOMERASE |
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| Author(s): SCHMIDT M, ARNOLD W, NIEMANN A, KLEICKMANN A, PUHLER A |
| Source: GENE Volume: 122 Issue: 1 Pages: 35-43 Published: DEC 1 1992 |
| Times Cited: 19 References: 59 |
| Abstract: Interspecific complementation of a Xanthomonas campestris pv. campestris phosphomannose isomerase (PMI) mutant was used to isolate a cosmid from a genomic library of Rhizobium meliloti 2011 carrying the pmi gene of this strain. Subcloning experiments localized the coding region to a 2.0-kb SalI-ClaI fragment. Nucleotide sequence analysis of this fragment indicated the presence of two open reading frames (ORFs), coding for 18- and 43-kDa polypeptides. The analysis of the gene function by gene disruption experiments showed that ORF2 codes for pmi. A comparison of the deduced amino acid sequence with the corresponding sequences of the Pseudomonas aeruginosa and Escherichia coli PMIs revealed no significant homology, indicating that the isolated gene encodes a new type of PMI. The construction of a pmi-deficient mutant of R. meliloti using the sacB-sacR cassette technique showed that the loss of PMI activity does not affect the symbiotic properties of this strain. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV BIELEFELD, FAK BIOL, LEHRSTUHL GENET, W-4800 BIELEFELD 1, GERMANY |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| Subject Category: Genetics & Heredity |
| IDS Number: KB098 |
| ISSN: 0378-1119 |