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| PURIFICATION AND CHARACTERIZATION OF A CHITOSANASE FROM STREPTOMYCES N174 |
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| Author(s): BOUCHER I, DUPUY A, VIDAL P, NEUGEBAUER WA, BRZEZINSKI R |
| Source: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY Volume: 38 Issue: 2 Pages: 188-193 Published: NOV 1992 |
| Times Cited: 51 References: 32 |
| Abstract: A highly efficient chitosanase producer, the actinomycete N174, identified by chemotaxonomic methods as belonging to the genus Streptomyces was isolated from soil. Chitosanase production by N174 was inducible by chitosan or D-glucosamine. In culture filtrates the chitosanase accounted for 50-60% of total extracellular proteins. The chitosanase was purified by polyacrylic acid precipitation, CM-Sepharose and gel permeation chromatography. The maximum velocity of chitosan degradation was obtained at 65-degrees-C when the pH was maintained at 5.5. The enzyme degraded chitosans with a range of acetylation degrees from 1 to 60% but not chitin or CM-cellulose. The enzyme showed an endo-splitting type of activity and the end-product of chitosan degradation contained a mixture of dimers and trimers of D-glucosamine. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV SHERBROOKE, FAC SCI, DEPT BIOL, RECH BIOL ACTINOMYCETES GRP, 2500 BLVD UNIV, SHERBROOKE J1K 2R1, QUEBEC CANADA
2. UNIV SHERBROOKE, FAC SCI APPL, DEPT GENIE CHIM, SHERBROOKE J1K 2R1, QUEBEC CANADA
3. NATL RES COUNCIL CANADA, DIV SCI BIOL, OTTAWA, ONTARIO CANADA |
| Publisher: SPRINGER VERLAG, 175 FIFTH AVE, NEW YORK, NY 10010 |
| Subject Category: Biotechnology & Applied Microbiology |
| IDS Number: KB406 |
| ISSN: 0175-7598 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |