| HEPARIN-BINDING KERATINOCYTE GROWTH-FACTOR IS A CANDIDATE STROMAL TO EPITHELIAL-CELL ANDROMEDIN |
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| Author(s): YAN GC, FUKABORI Y, NIKOLAROPOULOS S, WANG F, MCKEEHAN WL |
| Source: MOLECULAR ENDOCRINOLOGY Volume: 6 Issue: 12 Pages: 2123-2128 Published: DEC 1992 |
| Times Cited: 259 References: 31 |
| Abstract: The growth of isolated epithelial and stromal cells from both androgen-dependent normal rat prostate and an androgen-responsive model rat prostate tumor is androgen-independent. When added to cocultures of epithelial and stromal cells separated by a semipermeable membrane, androgen stimulated epithelial cell growth without an effect on stromal cell growth. Northern blot and nuclease protection analysis of mRNA revealed that stromal cells specifically expressed an androgen-sensitive secreted member of the heparin-binding fibroblast growth factor family [keratinocyte growth factor (KGF)/fibroblast growth factor-7]. KGF was mitogenic for epithelial cells, but not for stromal cells. Epithelial cells expressed specifically a splice variant of the bek receptor gene that specifically binds KGF. Expression of the bek receptor gene in stromal cells was undetectable by Northern blot and nuclease protection analyses. The results suggest that stromal cell-derived KGF has the properties of an andromedin, which mediates the indirect control of epithelial cell proliferation by androgen through a directional stromal-to-epithelial cell paracrine mechanism. |
| Document Type: Article |
| Language: English |
Addresses:
1. W ALTON JONES CELL SCI CTR, 10 OLD BARN RD, LAKE PLACID, NY 12946 USA |
| Publisher: ENDOCRINE SOC, 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 |
| Subject Category: Endocrinology & Metabolism |
| IDS Number: KE801 |
| ISSN: 0888-8809 |