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| MR 92,000 TYPE-IV COLLAGENASE IS INCREASED IN PLASMA OF PATIENTS WITH COLON CANCER AND BREAST-CANCER |
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| Author(s): ZUCKER S, LYSIK RM, ZARRABI MH, MOLL U |
| Source: CANCER RESEARCH Volume: 53 Issue: 1 Pages: 140-146 Published: JAN 1 1993 |
| Times Cited: 232 References: 41 |
| Abstract: Overproduction of matrix metalloproteinases (MMPs) is a common characteristic of metastatic cancer cells. Since MMPs can be identified in plasma, we proposed that enhanced MMP-9 secretion by invasive cancer cells may be detected by plasma assay. To this end, we developed a specific sandwich enzyme-linked immunosorbent assay which uses two mouse monoclonal antibodies to human M(r) 92,000 type IV collagenase (MMP-9). The plasma concentration of MMP-9 (mean +/- SD) in 60 healthy subjects (9 +/- 11 ng/ml), 136 patients without cancer, and 179 patients with cancer of the lung, genitourinary tract, or lymphomas-leukemias did not differ significantly. In contrast, plasma MMP-9 was significantly increased (P < 0.01) in 122 patients with gastrointestinal tract cancer and breast cancer (18 +/- 23 and 21 +/- 22 ng/ml, respectively). Whereas carcinoembryonic antigen levels were significantly increased in patients with stage IV gastrointestinal cancer, MMP-9 concentrations were not significantly increased in patients with metastatic disease as compared to those with nonmetastatic cancer. Combining both assays improves sensitivity of detection of colon cancer. MMP-9 was also significantly increased during pregnancy which is consistent with the extensive ongoing tissue remodeling and the leaching of the tissue proteinase into plasma. |
| Document Type: Article |
| Language: English |
| Reprint Address: ZUCKER, S (reprint author), DEPT VET AFFAIRS MED CTR, DEPT RES, MAIL CODE 151, NORTHPORT, NY 11768 USA |
Addresses:
1. DEPT VET AFFAIRS MED CTR, DEPT MED, NORTHPORT, NY 11768 USA
2. SUNY STONY BROOK, DEPT MED, STONY BROOK, NY 11794 USA
3. SUNY STONY BROOK, DEPT PATHOL, STONY BROOK, NY 11794 USA |
| Publisher: AMER ASSOC CANCER RESEARCH, PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 |
| Subject Category: Oncology |
| IDS Number: KD719 |
| ISSN: 0008-5472 |
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