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| CDNA CLONING OF A GERM-CELL SPECIFIC LAMIN-B3 FROM MOUSE SPERMATOCYTES AND ANALYSIS OF ITS FUNCTION BY ECTOPIC EXPRESSION IN SOMATIC-CELLS |
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| Author(s): FURUKAWA K, HOTTA Y |
| Source: EMBO JOURNAL Volume: 12 Issue: 1 Pages: 97-106 Published: JAN 1993 |
| Times Cited: 141 References: 67 |
| Abstract: The nuclear lamina is a fundamental component involved in the assembly of the nuclear envelope and higher order chromosomal structures in eukaryotes. In mammals, it is composed of four major lamin proteins, termed lamins A, B1, B2 and C. Here we first report cDNA cloning of a new 53 kDa lamin protein from mouse spermatocytes, termed lamin B3, the expression of which appears restricted to spermatogenic cells. Its gene structure indicates that lamin B3 is generated by differential splicing and alternative polyadenylation from lamin B2. When lamin B3 is introduced into somatic cells in culture, their nuclear morphology is transformed from spherical to hook-shaped. On the basis of the results obtained, we suggest that the germ cell specific lamin B3 is involved in the reorganization of nuclear and chromosomal structures during meiotic division. |
| Document Type: Article |
| Language: English |
| Reprint Address: FURUKAWA, K (reprint author), NAGOYA UNIV, SCH SCI, DEPT BIOL, NAGOYA 46401, JAPAN |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: KG621 |
| ISSN: 0261-4189 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |