| A POSITIVE ROLE FOR HISTONE ACETYLATION IN TRANSCRIPTION FACTOR ACCESS TO NUCLEOSOMAL DNA |
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| Author(s): LEE DY, HAYES JJ, PRUSS D, WOLFFE AP |
| Source: CELL Volume: 72 Issue: 1 Pages: 73-84 Published: JAN 15 1993 |
| Times Cited: 660 References: 75 |
| Abstract: Acetylation of the N-terminal tails of the core histones directly facilitates the recognition by TFIIIA of the 5S RNA gene within model chromatin templates. This effect is independent of a reduction in the extent of histone-DNA interactions or a change in DNA helical repeat; it is also independent of whether a histone tetramer or octamer inhibits TFIIIA binding. Removal of the N-terminal tails from the core histones also facilitates the association of TFIIIA with nucleosomal templates. We suggest that the histone tails have a major role in restricting transcription factor access to DNA and that their acetylation releases this restriction by directing dissociation of the tails from DNA and/or inducing a change in DNA configuration on the histone core to allow transcription factor binding. Acetylation of core histones might be expected to exert a major influence on the accessibility of chromatin to regulatory molecules. |
| Document Type: Article |
| Language: English |
| Reprint Address: LEE, DY (reprint author), NICHHD, MOLEC EMBRYOL LAB, BETHESDA, MD 20892 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: KG955 |
| ISSN: 0092-8674 |