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| CLONED HUMAN BRAIN NITRIC-OXIDE SYNTHASE IS HIGHLY EXPRESSED IN SKELETAL-MUSCLE |
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| Author(s): NAKANE M, SCHMIDT HHHW, POLLOCK JS, FORSTERMANN U, MURAD F |
| Source: FEBS LETTERS Volume: 316 Issue: 2 Pages: 175-180 Published: JAN 25 1993 |
| Times Cited: 448 References: 30 |
| Abstract: Complementary DNA clones corresponding to human brain nitric oxide (NO) synthase have been isolated. The deduced amino acid sequence revealed an overall identity with rat brain NO synthase of about 93% and contained all suggested consensus sites for binding of the co-factors. The cDNA transfected COS-1 cells showed significant NO synthase activity with the typical co-factor requirements. Unexpectedly, messenger RNA levels of this isoform of NO synthase was more abundant in human skeletal muscle than human brain. Moreover, we detected high NO synthase activity and the expressed protein in human skeletal muscle by Western blot analysis, indicating a possible novel function of NO in skeletal muscle. |
| Document Type: Article |
| Language: English |
| Reprint Address: NAKANE, M (reprint author), ABBOTT LABS, VASC BIOL, D46B, AP10, ABBOTT PK, IL 60064 USA |
Addresses:
1. NORTHWESTERN UNIV, SCH MED, DEPT PHARMACOL, CHICAGO, IL 60611 USA |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| Subject Category: Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| IDS Number: KH969 |
| ISSN: 0014-5793 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |