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| MOLECULAR-CLONING AND EXPRESSION OF A HUMAN ANTERIOR-PITUITARY RECEPTOR FOR GROWTH HORMONE-RELEASING HORMONE |
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| Author(s): GAYLINN BD, HARRISON JK, ZYSK JR, LYONS CE, LYNCH KR, THORNER MO |
| Source: MOLECULAR ENDOCRINOLOGY Volume: 7 Issue: 1 Pages: 77-84 Published: JAN 1993 |
| Times Cited: 165 References: 43 |
| Abstract: GH-releasing hormone (GHRH), acting through the GHRH receptor (GHRH-R), plays a pivotal role in the regulation of GH synthesis and secretion in the pituitary. It is possible that GHRH may serve other roles in other tissues. Here we report the cloning of a cDNA encoding a human GHRH-R from an acromegalic pituitary cDNA library. The isolated cDNA encodes a 423-amino acid protein that has seven putative transmembrane domains characteristic of G-protein-coupled receptors. It is a member of the secretin family of G-protein-coupled receptors and has 47%,42%,35%, and 28% identity with receptors for vasoactive intestinal peptide, secretin, calcitonin, and PTH, respectively. Transient expression of this cDNA in COS cells induced saturable, high affinity, GHRH-specific binding and also stimulated intracellular cAMP accumulation in response to physiological concentrations of GHRH. A specific GHRH antagonist blocked both binding and second messenger response. Northern analysis indicated that GHRH-R mRNA was most abundant in extracts of pituitary and was not detected in other tissues. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV VIRGINIA, SCH MED, HLTH SCI CTR, DEPT MED, BOX 511-66, CHARLOTTESVILLE, VA 22908 USA
2. UNIV VIRGINIA, SCH MED, DEPT PHARMACOL, CHARLOTTESVILLE, VA 22908 USA
3. AMER CYANAMID CO, DIV AGR RES, PRINCETON, NJ 08543 USA |
| Publisher: ENDOCRINE SOC, 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 |
| Subject Category: Endocrinology & Metabolism |
| IDS Number: KK295 |
| ISSN: 0888-8809 |
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