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| A FUNCTIONAL-ROLE FOR GTP-BINDING PROTEINS IN SYNAPTIC VESICLE CYCLING |
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| Author(s): HESS SD, DOROSHENKO PA, AUGUSTINE GJ |
| Source: SCIENCE Volume: 259 Issue: 5098 Pages: 1169-1172 Published: FEB 19 1993 |
| Times Cited: 108 References: 47 |
| Abstract: The squid giant synapse was used to test the hypothesis that guanosine-5'-triphosphate (GTP)-binding proteins regulate the local distribution of synaptic vesicles within nerve terminals. Presynaptic injection of the nonhydrolyzable GTP analog GTPgammaS irreversibly inhibited neurotransmitter release without changing either the size of the calcium signals produced by presynaptic action potentials or the number of synaptic vesicles docked at presynaptic active zones. Neurotransmitter release was also inhibited by injection of the nonhydrolyzable guanosine diphosphate (GDP) analog GDPbetaS but not by injection of AlF4-. These results suggest that a small molecular weight GTP-binding protein directs the docking of synaptic vesicles that occurs before calcium-dependent neurotransmitter release. Depletion of undocked synaptic vesicles by GTPgammaS indicates that additional GTP-binding proteins function in the terminal at other steps responsible for synaptic vesicle replenishment. |
| Document Type: Article |
| Language: English |
Addresses:
1. DUKE UNIV, MED CTR, DEPT NEUROBIOL, POB 3209, DURHAM, NC 27710 USA
2. UNIV SO CALIF, DEPT BIOL SCI, LOS ANGELES, CA 90089 USA
3. MARINE BIOL LAB, WOODS HOLE, MA 02543 USA
4. KIEV PHYSIOL INST, KIEV, UKRAINE
5. MAX PLANCK INST BIOPHYS CHEM, W-3400 GOTTINGEN, GERMANY |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: KM917 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |